Microbial Genetics Chapter 8
  1. Overview
    1. Genome
    2. Chromosome
    3. Gene
      1. functional units
        1. mRNA
        2. tRNA
        3. rRNA
    4. Central dogma of molecular biology
    5. Genotype
    6. Phenotype
  2. DNA
    1. Location
    2. Composition
    3. Structure review
      1. nucleotide
        1. deoxyribose
        2. phosphate group
        3. base
      2. double helix
    4. Replication
      1. helicasee
      2. DNA polymerase
      3. bidirectional
        1. antiparallel
      4. DNA ligase
      5. semiconservative replication
      6. accuracy
  3. Gene Expression
    1. Transcription
      1. RNA review
      1. The hows of Transcription
        1. Initiation
          1. RNA polymerase

              promoter
          2. gene structure
            1. operon
            2. polycistronic
        2. Elongation
        3. Termination
          1. terminator

            B. Translation
      2. components
      3. ribosomes
      4. tRNA
        1. folding
          1. cloverleaf
        2. charging
      5. Genetic code
        1. codons
      6. How Translation is done
        1. Initiation
          1. ribosome-binding site
          2. AUG
        2. Elongation
          1. P-site
          2. A-site
          3. E-site
        3. Termination
          1. stop codon
          2. UGA, UAG, UAA
  4. Mutation
    1. Types
      1. point mutation
        1. silent
        2. neutral
        3. missense
        4. nonsense
      2. frameshift
    2. Causes
      1. spontaneous
      2. mutagens
        1. thymine dimer
    3. Identifying mutants
      1. positive (direct) selection
      2. negative (indirect) selection
        1. auxotroph
      3. Ames test
  5. Genetic Recombination
    1. Transformation
      1. Frederick Griffith
    2. Conjugation
    3. Transduction