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Laboratory Techniques |
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General Class Resources:
Syllabus
Proposed Course Schedule
- Beginning Lecture Material
- Syllabus & about the class ✓
- Safety ✓
- Pipets & Micropipets ✓
- Solutions & Mixing
- pH meter calibration and use
- Making pHed solutions
- Make media - autoclave and plate pouring ✓
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- LB Media Recipe
- Nice LB media web page
- Culturing environmental bacteria
- DNA isolation lecture
- DNA quantitation lecture
- Isolate environmental bacterial DNA
- MoBIO Power Biofilm DNA Isolation kit Manual
- MoBIO Power Lyzer Ultra Clean Microbial DNA Isolation kit Manual
- PCR
- PCR & primers lecture
- Run PCR
- PCR electrophoresis
- Plasmids
- Making plates with antibiotic
- Culturing bacteria from stock
- Plasmids lecture
- Restriction enzymes lecture
- DNA staining lecture
- Isolate plasmids
- Cut plasmids
- Pouring agarose gels
- Electrophorses plasmids
- Tick 'gathering' in October
- DNA isolation
- PCR
- Laboratory Resources:
- Solution Calculations Practice
- Solution Calculations Practice Answers
- Tissue Culture Container Areas
- Tissue Culture Passaging Procedure
- Company Links:
- New Englend BioLabs
- Promega
- Bio-Rad
- Fisher Scientific
- Databases:
- Genbank
- PubMed
- EzTaxon
- Primer Tm Calculators:
- Promega
- NEB
- Online PCR Primer Designs
- OligoPerfect Designer
- Primer-BLAST
- Primer3Plus
- Additional Online Resources
- Centrifuge Rotor Speed Calculator
- Tissue Culture Info
- Fun Video Links:
- Gangnam Style Biology
- Protein Purification Blues
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