Laboratory Techniques


General Class Resources:

Syllabus


Proposed Course Schedule

Beginning Lecture Material
Syllabus & about the class ✓
Safety ✓
Pipets & Micropipets ✓
Micropipet Settings Practice Sheet
Solutions & Mixing
Solution Calculations Practice
Solution Calculations Practice Answers
pH meter calibration and use
Making pHed solutions
Make media - autoclave and plate pouring ✓
LB Media Recipe
Nice LB media web page
Culturing environmental bacteria
DNA isolation lecture ✓
DNA quantitation lecture ✓
Isolate environmental bacterial DNA
MoBIO Power Biofilm DNA Isolation kit Manual
MoBIO Power Lyzer Ultra Clean Microbial DNA Isolation kit Manual
PCR
PCR & primers lecture
Run PCR ✓
Agarose electrophoresis ✓
Plasmids
Making plates with antibiotic
Culturing bacteria from stock ✓
Plasmids lecture ✓
Restriction enzymes lecture
DNA staining lecture
Isolation of Plasmids
Biol Prep ✓
Promega PureYield Plasmid Miniprep System Quick Protocol
Promega PureYield Plasmid Miniprep System Manual
Cut plasmids
Pouring agarose gels ✓
Electrophorses plasmids ✓
Tick 'gathering' in October
DNA isolation
PCR

Laboratory Resources:
Tissue Culture Container Areas
Tissue Culture Passaging Procedure
Company Links:
New Englend BioLabs
Promega
Bio-Rad
Fisher Scientific
Databases:
Genbank
PubMed
EzTaxon
Primer Tm Calculators:
Promega
NEB
Online PCR Primer Designs
OligoPerfect Designer
Primer-BLAST
Primer3Plus
Additional Online Resources
Centrifuge Rotor Speed Calculator
Tissue Culture Info
Fun Video Links:
Gangnam Style Biology
Protein Purification Blues

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