 |
Laboratory Techniques |
|  |
General Class Resources:
Syllabus
Proposed Course Schedule
- Beginning Lecture Material
- Syllabus & about the class ✓
- Safety ✓
- Pipets & Micropipets ✓
- Micropipet Settings Practice Sheet
- Solutions & Mixing
- Solution Calculations Practice
- Solution Calculations Practice Answers
- pH meter calibration and use
- Making pHed solutions
- Make media - autoclave and plate pouring ✓
-
- LB Media Recipe
- Nice LB media web page
- Culturing environmental bacteria
- DNA isolation lecture ✓
- DNA quantitation lecture ✓
- Isolate environmental bacterial DNA
- MoBIO Power Biofilm DNA Isolation kit Manual
- MoBIO Power Lyzer Ultra Clean Microbial DNA Isolation kit Manual
- PCR
- PCR & primers lecture
- Run PCR ✓
- Agarose electrophoresis ✓
- Plasmids
- Making plates with antibiotic
- Culturing bacteria from stock ✓
- Plasmids lecture ✓
- Restriction enzymes lecture
- DNA staining lecture
- Isolation of Plasmids
- Biol Prep ✓
- Promega PureYield Plasmid Miniprep System Quick Protocol
- Promega PureYield Plasmid Miniprep System Manual
- Cut plasmids
- Pouring agarose gels ✓
- Electrophorses plasmids ✓
- Tick 'gathering' in October
- DNA isolation
- PCR
- Laboratory Resources:
- Tissue Culture Container Areas
- Tissue Culture Passaging Procedure
- Company Links:
- New Englend BioLabs
- Promega
- Bio-Rad
- Fisher Scientific
- Databases:
- Genbank
- PubMed
- EzTaxon
- Primer Tm Calculators:
- Promega
- NEB
- Online PCR Primer Designs
- OligoPerfect Designer
- Primer-BLAST
- Primer3Plus
- Additional Online Resources
- Centrifuge Rotor Speed Calculator
- Tissue Culture Info
- Fun Video Links:
- Gangnam Style Biology
- Protein Purification Blues
-
| classes |